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. 2011 Apr 15;25(8):831–844. doi: 10.1101/gad.615311

Figure 1.

Figure 1.

Vegfr3:YFP expression in the adult SVZ. (Top left panel) The localization of Vegfr3:YFP cells (green) around the lv is schematically represented. All images are taken in the lateral ventricular wall facing the striatum (St). Confocal (A–E) and electron microscopy (F,G) images from Vegfr3:YFP mouse brain vibratome sections are stained with Abs against cell type-specific markers as indicated. (A) Vegfr3:YFP cells are immunolabeled by anti-VEGFR-3 Ab (yellow arrows), indicating that YFP faithfully reports VEGFR-3 expression in SVZ cells. (B) Vegfr3:YFP cells are a subpopulation of GFAP+ cells (yellow arrows), extending long cytoplasmic processes into the SVZ. (C) Few Vegfr3:YFP cells are labeled by anti-EGFR Ab (yellow arrow), but most of the EGFR+ cells are YFP-negative. YFP is expressed by few vessels in the striatum outside the SVZ (white arrow). (D) The majority of BrdU LRCs along the striatal SVZ are Vegfr3:YFP cells (yellow arrows). (E) Vegfr3:YFP does not label DCX+ neuroblasts (red). (White arrow) Blood vessel. (F,G) Immunogold labeling of Vegfr3:YFP cells in the ventricular zone–SVZ. (F) A labeled type B1 NSC extending a primary cilium (magnification in inset). (G) A blood vessel (bv, YFP-negative) is enwrapped by the cytoplasmic process of a Vegfr3:YFP astrocyte (asterisk). (Arrows) Basal laminae. Bars: A–E, 10 μm; F, 2 μm; G, 500 nm.