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Limited proteolysis analysis on RHBV NS3 treated by chymotrypsin. (Lanes 1–5) Full-length RHBV NS3 protein incubated in different concentrations of chymotrypsin varying from 2 to 0.125 μM for 5 min. (Lane 6) Full-length NS3 protein without subjected to enzyme digestion as a control. (Lane 7) Perfect protein marker.
