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. 2011 May;17(5):933–943. doi: 10.1261/rna.2533811

FIGURE 1.

FIGURE 1.

Anti-miRs can interfere with miR-122 detection by Northern blot. (A) Northern blot of total purified RNA from Huh7 cells treated with miR-122 anti-miRs K-PNA-K3, OMe, LNA/OMe, or LNA/DNA. Total purified RNA from untreated cells was used as negative controls. (B) Northern blot of total purified RNA after addition of miR-122 anti-miRs at equivalent amounts to mimic transfections in A to Huh7 cell lysates. Total purified RNA from Huh7 cells lysates without addition of anti-miR was used as negative control. (C) Northern blot of total RNA from Huh7 cell lysates treated as in B with OMe, LNA/OMe, or LNA/DNA anti-miR and further addition of 100 pmol of decoy PNA ON to the purified RNA. RNA from untreated lysates but with 100 pmol of decoy PNA ON addition was used as negative control. U6 RNA was used as loading control for all gels.