FIGURE 3.

miR-122 loss and recovery during RNA extraction. Denaturing PAGE of cell lysates supplemented with ³²P-miR122 and further addition of K-PNA-K3, OMe, LNA/OMe, or LNA/DNA anti-miRs before RNA extraction, at equivalent amounts to mimic transfections in Figure 1A. Negative controls correspond to cell lysates spiked with ³²P-miR122 only. (A) Purified RNA (upper panel) and the remaining isopropanol supernatant (lower panel) of samples after RNA extraction with 1 equivalent (v/v) isopropanol. (B) Purified RNA (upper panel) and the remaining isopropanol supernatant (lower panel) from cell lysates treated as in A with LNA/OMe anti-miR after RNA extraction with 1, 2, or 3 equivalents (v/v) isopropanol. (C) Purified RNA from samples treated as in A after RNA extraction with 3 equivalents (v/v) isopropanol.