FIGURE 4.

miRNA fate upon targeting with LNA/DNA, LNA/OMe, or OMe anti-miR. (A) Northern blot of total purified RNA extracted with 1 equivalent (v/v) isopropanol from Huh7 cells treated as in Figure 1A and further addition of decoy PNA ON to the RNA samples before PAGE separation. RNA from untreated Huh7 cells but in the presence of decoy PNA ON was used as negative control. (B–D) Northern blots of total purified RNA extracted with 3 equivalents (v/v) isopropanol from HuH7 cells treated as in A with either LNA/DNA anti-miR (B), LNA/OMe anti-miR (C), or OMe anti-miR (D) in the presence or absence of decoy PNA ON. RNA from untreated Huh7 cells was used as negative control. U6 RNA was used as loading control for all gels.