FIGURE 2.

Cap dependence of TCE-mediated repression. (A) Reporter RNAs used in B and C are similar to those in Figure 1 and differ from each other by the presence of an m⁷GpppG cap (m⁷G) or an ApppG cap (Acap). 3′UTR* denotes tub or 3xTCE (TCE) sequences. (B,C) Luciferase assays of the m⁷G and Acap luc-tub3′UTR (tub) and luc-3xTCE (TCE) reporter RNAs in late ovary or embryo extract. Relative luciferase activity was determined as described in Figure 1, except that the normalized luciferase activity of each luc-3xTCE RNA was divided by the normalized activity of its cognate luc-tub3′UTR RNA (for each, tub = 1.0).