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. 2011 Mar 18;90(2):353–363. doi: 10.1093/cvr/cvq410

Figure 4.

Figure 4

Endothelial PKG1 downregulation directly regulates NOS3 activity. Data were performed using BAECs and are expressed as percentage of untransfected cells. (A) Representative immunoblot probed for PKG1β Ab following infection with PKG-siRNA, with corresponding densitometry quantification, in the absence or presence of 8Br-cGMP. (B) PKG activity following EC transfection with PKG1-siRNA, in the absence or presence of 8Br-cGMP. (C) Immunoblot probed for PKG1β, P-NOS3(S1179), and total NOS3 following infection with siRNA for PKG1 and corresponding blot quantification P/Total-NOS. (D) Calcium-dependent radiolabelled NOS activity following transfection with siRNA for PKG1 in the absence of presence of 8Br-cGMP.