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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Dev Cell. 2010 Jun 15;18(6):913–926. doi: 10.1016/j.devcel.2010.05.017

Figure 1.

Figure 1

CDK5RAP2 is a centrosomal protein whose levels change with the cell cycle (A) Schematic comparing Drosophila centrosomin (CNN), with human and mouse CDK5RAP2 proteins. Two conserved domains, each about 60 amino acids in length, are designated CNN motifs 1 (CM1) and 2 (CM2). The black line indicates the region used to raise antibodies. The identities and similarities between CNN and mCDK5RAP2 within CM1 and CM2 are shown. In addition to these conserved blocks, CNN family members contain extensive coiled-coil regions. (B) Immunofluorescence images of NIH-3T3 mouse fibroblasts stained for CDK5RAP2 (red), the microtubule marker α-tubulin (green), and DNA (blue) at different stages of the cell cycle. Insets are enlargements of CDK5RAP2 signal to highlight changes in CDK5RAP2 levels at centrosomes. Scale bar = 10 μm. See also Figure S1.