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. 2011 Apr 12;124(9):1486–1495. doi: 10.1242/jcs.082834

Fig. 2.

Fig. 2.

Truncated MCP1 compromises BBB integrity. (A) The indicated form of MCP1 (100 nM) was added to the lower chamber of the BMEC-astrocyte co-culture system. TEER values were determined over time in the presence of A2AP. Results are means±s.d. (n=3). Ctr, control (saline). (B) Cells were treated as in A. The leakage of Evans Blue across the co-culture system was determined spectrophotometrally after treatment with A2AP. Results are means±s.d. (n=4). *P<0.05 (analyzed using one-way ANOVA followed by Newman–Keuls multiple comparison test) compared with results with FL-MCP1. (C) Infiltration of Evans Blue across the compromised BBB into brain parenchyma was assayed 12 hours after a single injection of MCP1 (1 μg). Mice injected with saline were used as control. Results [OD (optical density; absorbance) per g of body weight] are means+s.d. (n=4). *P<0.05 (analyzed using one-way ANOVA followed by Newman–Keuls multiple comparison test) compared with results with FL-MCP1 without A2AP.