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. 2011 Apr 12;124(9):1486–1495. doi: 10.1242/jcs.082834

Fig. 7.

Fig. 7.

Truncated MCP1 promotes interaction between phosphorylated ERM proteins and ZO-1. Mouse BMECs were treated with K104Stop-MCP1 or saline (0 h) for 1 hour. Cell lysates were collected and used for co-immunoprecipitation experiments. (A) Immunoprecipitation (IP) with an anti-(phosphorylated ERM) antibody (p-ERM) and immunoblotting (IB) with anti-ZO-1 and anti-occludin antibodies. (B) Immunoprecipitation with an anti-ZO-1 antibody and immunoblotting with anti-(phosphorylated ERM) and anti-occludin antibodies. (C) Immunoprecipitation with an anti-occludin antibody and immunoblotting with anti-(phosphorylated ERM) and anti-ZO-1 antibodies. Anti-(mouse IgG) antibody was used as control (Ctr).