Fig. 1.

Distribution of retromer components across eukaryotic lineages. Most retromer components are conserved across eukaryotes, except Vps17, which is restricted to opisthokonts. Vps5 and Vps10 are frequently lost. The factors are named using S. cerevisiae nomenclature. Large taxon groupings are colour coded, and a diagram defining the species represented is given. Data are based on BLAST and HMMer results together with alignments and phylogenetics. Filled sectors indicate the presence of the protein (see Materials and Methods) and white sectors indicate that the protein was not found. The numbers in the sectors indicate that multiple copies of factors were found (the products of distinct genes). The asterisks in the Vps26 column indicate the presence of a DSCR3 homologue and a Vps26-encoding gene. The accession numbers are given in supplementary material Table S1. Species abbreviations: Opisthokonts (blue): Hs, Homo sapiens; Dm, Drosophila melanogaster; Ce, Caenorhabditis elegans; Dr, Danio rerio; Nv, Nematostella vectensis; Mb, Monosiga brevicollis; Sc, Saccharomyces cerevisiae; Cn, Cryptococcus neoformans; An, Aspergillus nidulans; Sp, Schizosaccharomyces pombe; Ro, Rhizopus oryzae. Amoebozoa (purple): Eh, Entamoeba histolytica; Dd, Dictyostelium discoideum. Archaeplastida (green): At, Arabidopsis thaliana; Os, Oryza sativa; Pt, Populus trichocarpa; Cr, Chlamydomonas reinhardtii; Ot, Ostreococcus tauri; Cm, Cyanidioschyzon merolae. Crypto- and Hapto-phyta (bronze): Gt, Guillardia theta; Em, Emiliania huxleyi. Stramenopile-Alveolate-Rhizaria (i.e. SAR) (orange): Pf, Plasmodium falciparum; Tg, Toxoplasma gondii; Cp, Cryptosporidium parvum; Tpa, Theileria parva; Tt, Tetrahymena thermophila; Tps, Thalassiosira pseudonana; Pr, Phytophthora ramorum; Bn, Bigelowiella natans. Excavata (fuchsia): Tb, Trypanosoma brucei; Lm, Leishmania major; Ng, Naegleria gruberi; Tv, Trichomonas vaginalis; Gi, Giardia intestinalis. This convention is used throughout.