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. 2011 Apr 18;124(9):1581–1588. doi: 10.1242/jcs.073171

Fig. 2.

Fig. 2.

P2X7 is involved in Thy-1-induced FA formation. (A) Confocal images of FA (green or yellow) and stress fibers (red). Non-stimulated astrocytes (NS) or astrocytes stimulated with either Thy-1(RLD)-Fc–Protein A (Thy-1) for 15 minutes, or incubated with BzATP (100 μM) for 15 minutes, or pre-incubated with oATP (600 μM) and then stimulated with Thy-1(RLD)-Fc–Protein A for 15 minutes (Thy-1+oATP) are shown. Scale bar: 25 μm. (B,C) Results from A, which also included treatment with 2 mM EGTA for 15 minutes (only shown in B and C) were quantified (n=3). The number of FA per cell (B) and the area per FA (C) are shown. In each experiment, values for at least 50 different cells were averaged per condition (mean + s.e.m.); P<0.05 compared to control (*) or to Thy-1-stimulated samples (#).