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. 2011 Mar 24;11:26. doi: 10.1186/1472-6750-11-26

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Copyright ©2011 Jimenez et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Luciferase activity in stable lymphoid and epithelial transfectants carrying recombinant luciferase constructs. Luciferase activity was assessed in protein extracts from Daudi and HeLa cells with stable expression of monocistronic (tCD2-luc2) or bicistronic (luc2-IRES-tCD2) luciferase constructs. The substrate was D-luciferin (Promega luciferase assay system, Promega). Light measurements were done on a Microlumat LB96P luminometer (Berthold, Thoiry, France). This experiment was repeated thrice; error bars represent SD of three experiments (n = 3). Error bars are not apparent above rectangles corresponding to Daudi luc2-IRES-tCD2 and Hela tCD2-luc2 because experimental data for these conditions were very consistent (very small error bars are not apparent for this size of the graph using the Microsoft Excel software).