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qRT-PCR Conglutin Expression. Relative expression of conglutin genes determined by qRT-PCR using specific primers for (A) ALPHA, (B) BETA, (C) GAMMA and (D) DELTA conglutin sequences normalised to β-tubulin. RNA was extracted from L. angustifolius seeds collected from different stages of development. The average and standard error of three biological replicates are plotted against the log of the relative transcript expression.
