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. 2011 Mar 24;12:23. doi: 10.1186/1471-2172-12-23

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Copyright ©2011 van Maren et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of HKLP titration on proliferation of fresh versus cultured Teff cells and cultured Tregs. Cells were stimulated with different concentrations of HKLP in the absence or presence of anti-CD3. After 4 days proliferation was determined by analyses of thymidine incorporation (A) or CFSE dilution, displayed as MFI values (B). Cultured Treg proliferated in response to HKLP in a dose-dependent manner. Freshly isolated Teff also slightly responded to HKLP, while no effects were observed in cultured Teff. Representative results of 3 independent experiments are shown. The error bars represent standard deviations of duplicate measurements.