Figure 5.

CD16 expression on monocytes fed with different types of haemozoin in-vitro. PBMCs were isolated from blood of a healthy human volunteer and were incubated with synthetic Hz (sHz) at 0.004 and 0.007 μmoles/ml heme equivalent and with Plasmodium falciparum Hz (P.f.Hz) at 0.002 and 0.004 μmol/ml heme equivalent. PBMCs incubated with latex beads and with no Hz were used as controls. Cells were incubated for 6 hours and then labelled with anti-CD14 and anti-CD16. The Geometric mean values for expression of CD16 were calculated for non-Hz containing monocytes (open bars) and Hz-containing monocytes (grey bars). Differences between sHZ and P.f. were significant (P < 0.01). Shown are values of triplicates (± one SD). Controls and beads had no depolarizing populations.