Figure 7.

Detection of haemozoin in P. berghei ANKA infected mouse RBC. Uninfected control (upper panel). Whole blood from a C57BL/6 mouse with a parasitaemia of 8.1% (lower panel). Gate to indentify the depolarizing events in the control (0.2% events) (B) and in the infected mouse RBC (4.7% events) (D). Depolarizing events in this gate (D) were indentified as erythrocytes by anti-TER119 staining (> 97% positive) and are shown as red dots in E. Colours in plots A-D represent frequency of events. In plot E colour backgating; with blue = TER119 positive events without depolarization, red = TER119 positive events which depolarize; and green = any other event