Table 3. Percentages of responsive genes modulated by PPAR agonists in primary human hepatocytes and in HepaRG cells.
Compound | Concentration (µM) | Common modulated genes (%) | ||||||
Primary human hepatocytes | HepaRG cells | |||||||
Number of donors | Number of passages | |||||||
1 | 2 | 3 | 4 | 1 | 2 | 3 | ||
TRO | 5 | 100.0 | 30.4 | 4.2 | 0.3 | 100.0 | 27.1 | 13.4 |
20 | 100.0 | 37.6 | 5.9 | 0.8 | 100.0 | 33.4 | 14.7 | |
40 | 100.0 | 56.2 | 31.3 | |||||
ROSI | 50 | 100.0 | 21.2 | 7.2 | 2.5 | 100.0 | 44.4 | 24.4 |
100 | 100.0 | 39.8 | 16.4 | 5.4 | 100.0 | 57.4 | 32.0 | |
150 | 100.0 | 52.4 | 27.7 | 11.4 | 100.0 | 68.4 | 45.9 | |
MURA | 50 | 100.0 | 33.8 | 9.5 | 2.5 | 100.0 | 36.5 | 17.6 |
100 | 100.0 | 45.9 | 20.3 | 6.0 | 100.0 | 53.9 | 30.1 | |
150 | 100.0 | 64.8 | 37.2 | 14.3 | 100.0 | 75.8 | 58.7 | |
TESA | 300 | 100.0 | 37.7 | 9.2 | 2.5 | 100.0 | 36.2 | 16.3 |
Percentages of responsive genes modulated by PPAR agonists in primary human hepatocytes from one to four donors and in one to three passages of HepaRG cells.