Table 5. Validation by qPCR analysis of the microarray results in HepaRG cells exposed to PPAR agonists.
Gene name | qPCR results | Microarray results | ||||||||
TRO5 µM | TRO 20 µM | ROSI 20 µM | MURA 20 µM | TESA 300 µM | TRO 5 µM | TRO 20 µM | ROSI 20 µM | MURA 20 µM | TESA 300 µM | |
FABP4 | 12.9 | 24.1 | 45.8 | 41.2 | 73.9 | 6.5 | 8.0 | 2.7 | 13.5 | 15.7 |
PDK4 | 1.1 | 2.9 | 9.6 | 4.7 | 8.3 | 1.3 | 1.7 | 4.2 | 1.1 | 20.0 |
FABP1 | 2.2 | 2.6 | 2.1 | 6.1 | 4.5 | 1.7 | 1.4 | 1.3 | 2.0 | 2.8 |
ADFP | 2.6 | 2.8 | 2.7 | 3.8 | 5.6 | 2.4 | 2.9 | 3.9 | 1.6 | 8.6 |
CYP3A4 | 2.5 | 4.1 | 16.5 | 3.9 | 7.9 | 2.5 | 3.3 | 4.4 | -1.2 | 2.9 |
CYP2B6 | 1.5 | 1.9 | 3.7 | -1.1 | -1.0 | 1.3 | 1.1 | 1.3 | -1.1 | 1.1 |
HMOX1 | 1.4 | 1.7 | -1.1 | -1.0 | 1.3 | 1.4 | 1.6 | 1.8 | 1.4 | 3.3 |
ALB | 1.4 | 0.9 | 1.3 | 1.4 | 1.9 | 1.0 | -1.4 | -1.6 | -1.0 | -1.2 |
ALD-B | -1.3 | 0.6 | -1.4 | -1.4 | -2.0 | -1.2 | -1.8 | -1.9 | -1.1 | -2.1 |
PPARα | -1.3 | 0.7 | 1.4 | 1.8 | -1.2 | 1.0 | 1.0 | -1.3 | 1.1 | -1.2 |
PPARγ1 | 1.9 | 1.3 | -1.1 | -1.7 | -1.5 | -1.1 | -1.1 | -1.1 | 1.0 | -1.0 |
mRNA fold change for each gene corresponds to the ratio of mRNA expression in HepaRG cells treated by PPAR agonists for 24h vs their untreated counterparts; the values are means of at least two microarrays or three qPCR experiments.