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. 2011 Apr 18;6(4):e18816. doi: 10.1371/journal.pone.0018816

Table 5. Validation by qPCR analysis of the microarray results in HepaRG cells exposed to PPAR agonists.

Gene name qPCR results Microarray results
TRO5 µM TRO 20 µM ROSI 20 µM MURA 20 µM TESA 300 µM TRO 5 µM TRO 20 µM ROSI 20 µM MURA 20 µM TESA 300 µM
FABP4 12.9 24.1 45.8 41.2 73.9 6.5 8.0 2.7 13.5 15.7
PDK4 1.1 2.9 9.6 4.7 8.3 1.3 1.7 4.2 1.1 20.0
FABP1 2.2 2.6 2.1 6.1 4.5 1.7 1.4 1.3 2.0 2.8
ADFP 2.6 2.8 2.7 3.8 5.6 2.4 2.9 3.9 1.6 8.6
CYP3A4 2.5 4.1 16.5 3.9 7.9 2.5 3.3 4.4 -1.2 2.9
CYP2B6 1.5 1.9 3.7 -1.1 -1.0 1.3 1.1 1.3 -1.1 1.1
HMOX1 1.4 1.7 -1.1 -1.0 1.3 1.4 1.6 1.8 1.4 3.3
ALB 1.4 0.9 1.3 1.4 1.9 1.0 -1.4 -1.6 -1.0 -1.2
ALD-B -1.3 0.6 -1.4 -1.4 -2.0 -1.2 -1.8 -1.9 -1.1 -2.1
PPARα -1.3 0.7 1.4 1.8 -1.2 1.0 1.0 -1.3 1.1 -1.2
PPARγ1 1.9 1.3 -1.1 -1.7 -1.5 -1.1 -1.1 -1.1 1.0 -1.0

mRNA fold change for each gene corresponds to the ratio of mRNA expression in HepaRG cells treated by PPAR agonists for 24h vs their untreated counterparts; the values are means of at least two microarrays or three qPCR experiments.