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The effect of pH on the affinity of CsOxOx for oxalate. A: Scheme showing the dissociation constants for the three protonation states of oxalate binding to unprotonated enzyme (E) and protonated enzyme (EH+). B: pH dependence on kinetic parameters Vmax/Km (•) and Vmax (▲) for the CsOxOx catalyzed reaction. The curves were generated by a fit to equations 2 and 3, respectively.
