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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Plant J. 2011 Feb 21;66(2):293–305. doi: 10.1111/j.1365-313X.2011.04491.x

Figure 5. Transient expression of exogenous genes in cotton protoplasts.

Figure 5

a. Subcellular localization of GFP proteins in cotton protoplasts. The protoplasts were transfected with indicated constructs and incubated for 12 hr before GFP observation. Arrows indicate the Hoechst 33342 staining of nucleus, confirming the nuclear localization of Axr2-GFP and WRKY28-GFP. b. Expression of luciferase reporter gene in cotton protoplasts. The protoplasts were transfected with 35S-LUC or UBQ-LUC, and the luciferase activity was detected 6 hr after transfection by a plate reader. The protoplasts were isolated from the cotyledons of cultivar FM9160B2F. The experiments were repeated three times with similar results. The data in (b) are shown as means ± standard errors from three independent biological replicates.