Table 1.
The efficiency of cotton protoplast isolation.
| Cultivar | Tissue | Age (week) |
Protoplast yield /ml enzyme solution |
Incubation time |
Protoplast size |
|---|---|---|---|---|---|
| Cropland Genetics 3035RF | Green cotyledon | 1 | 3 ×105 | 12 hr | Medium |
| Cropland Genetics 3035RF | Green cotyledon | 2 | 7.5 ×105 | 12 hr | Big |
| Cropland Genetics 3035RF | Green cotyledon | 2 | 0.64 ×105 | 3 h | Big |
| Cropland Genetics 3035RF | True leaf | 3 | 2.34 ×105 | 12 hr | Small |
| Cropland Genetics 3035RF | Etiolated cotyledon | 1 | 8.4 ×105 | 12 hr | Small |
| Cropland Genetics 3035RF | Etiolated cotyledon | 2 | 0.04 ×105 | 3 h | Small |
| FM9160B2F | Green cotyledon | 1 | 3.4 ×105 | 12 hr | Medium |
| FM9160B2F | Green cotyledon | 2 | 4 ×105 | 12 hr | Big |
| FM9160B2F | Green cotyledon | 2 | 1.16 ×105 | 3 hr | Big |
| FM9160B2F | True leaf | 3 | 13.2 ×105 | 12 hr | Small |
| FM9160B2F | Etiolated cotyledon | 1 | 5.4 ×105 | 12 hr | Small |
| FM9160B2F | Etiolated cotyledon | 2 | 0.08 ×105 | 3 hr | Small |