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. Author manuscript; available in PMC: 2012 Apr 12.
Published in final edited form as: Vaccine. 2011 Feb 23;29(17):3276–3283. doi: 10.1016/j.vaccine.2011.02.023

Figure 1. Molecular characterization of MVA35Δ.

Figure 1

a) PCR. MVA-infected cells were transfected with a recombinant PCR fragment containing the E. coli gpt gene inserted between the A35 flanking regions and recombinant viruses were selected in mycophenolic acid-containing media. Virus crude stocks were PCR analyzed using primers in the A35 flanking regions. Wild type A35 locus yields a product of 1400 kbp size and the mutants with gpt inserted yield a size of approx 1900 kbp. L=ladder, b) Western blot showing that A35 is not expressed in MVA35Δ-infected cells. BHK-21 cells were infected with listed viruses at an MOI of 20 for 2 h and analyzed by SDS-PAGE. Blots were incubated with rabbit anti-A35 antibody at a 1:1000 dilution and developed with an anti-rabbit alkaline phosphatase-conjugated secondary antibody.