Skip to main content
. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Clin Cancer Res. 2011 Jan 17;17(8):2361–2372. doi: 10.1158/1078-0432.CCR-10-2262

Figure 2. Smac mediates gemcitabine-induced apoptosis through the mitochondrial pathway.

Figure 2

(A) Bcl-2 suppressed gemcitabine-induced apoptosis. JHU-012 or 1483 cells were transfected with a Bcl-2 expression construct or empty vector followed by 50 μM gemcitabine treatment for 48 h, respectively. Upper, the expression of Bcl-2 was analyzed by Western blotting. Lower, apoptosis was analyzed by nuclear fragmentation assay. **P<0.01, Bcl-2 vs. vector or mock (un) transfected group. (B) Cytochrome c release in parental and SMAC-KD cells treated as in (A) was analyzed by Western blotting in the cytosolic fractions. Tubulin was used as control for loading. (C) Activation of caspases 3, 8 and 9 was analyzed by Western blotting in indicated cells treated with 50 μM gemcitabine for 48 h. (D) Left, Mitochondrial membrane depolarization in JHU-012 parental and SMAC-KD cells was analyzed by flow cytometry 48 h after 50 μM gemcitabine treatment. Right, quantitation of depolarized cells