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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Feb 18;1808(6):1701–1708. doi: 10.1016/j.bbamem.2011.02.009

Figure 3. Destabilization of microtubules abolishes cAR1 movement.

Figure 3

(A) Trajectory of a cAR1 molecule after 10 min incubation with 50 μM Benomyl (n=19 cells), the insert shows the trajectory on a smaller (10x) scale. (B) After destabilization of the microtubules with Benomyl there was only one population of cAR1-Halo-TMR molecules and when compared to the slow population of cAR1-Halo-TMR molecules (black squares), these were all completely immobile after addition of Benomyl (black circles). (C) Microtubules were visualized with tubulin-GFP. (D) After 10 min of incubation with 50 mM Benomyl the aster-like assemblies of microtubules were no longer observed. The microtubules were decomposed and a homogeneous distribution of tubulin-GFP could be seen in the cells. Fluorescence recovery curves of the lipid dye FM4-64 (E) and cAR1-eYFP (F) before (black) and after Benomyl addition (red) (n>10 cells for each condition). The recovery of the fluorescence after photobleaching within a circle of 1μm diameter of the basal plasma membrane is plotted against time. Different time intervals were used for FM4-64 and cAR1-eYFP.