Table 1.
Quantitation of total and SIV Env-specific IgG and IgA memory B cells in PBMC of rhesus macaquesa.
| Total IgG ASC | Total IgA ASC | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Unstimulated PBMC | Stimulated PBMC | Unstimulated PBMC | Stimulated PBMC | |||||||
| Days of culture | 0 | 3 | 5 | 3 | 5 | 0 | 3 | 5 | 3 | 5 |
| All Macaques | 788 ± 219 | 476 ± 182 | 541 ± 173 | 16852 ± 2799 | 23354 ± 3646 | 90 ± 38 | 85 ± 33 | 100 ± 31 | 991 ± 128 | 1446 ± 507 |
| Env-Specific IgG ASC | Env-Specific IgA ASC | |||||||||
| Naïve Macaques | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Unvaccinated infected | 1 ± 1 | 13 ± 7 | 40 ± 31 | 296 ± 46 | 305 ± 49 | 0 | 0 | 2 ± 2 | 15 ± 8 | 38 ± 28 |
| Vaccinated controllers | 2 ± 2 | 10 ± 8 | 49 ± 41 | 637 ± 293 | 311 ± 107 | 0 | 1 ± 1 | 6 ± 3 | 281 ± 106 | 82 ± 19 |
| IgG Specific Activity | IgA Specific Activity | |||||||||
| Naïve Macaques | 0 | 0 | 0 | 0 | ||||||
| Unvaccinated infected | 1.3 ± 0.3 | 0.9 ± 0.2 | 0.9 ± 0.5 | 3.4 ± 2.4 | ||||||
| Vaccinated controllers | 5.4 ± 0.8 | 2.4 ± 0.2 | 43.1 ± 11.6 | 30.7 ± 10.8 | ||||||
a
Total and Env-specific IgG and IgA ASC were enumerated by ELISPOT as described in materials and Methods. IgG and IgA specific activity was calculated as (Env-specific ASC /total memory ASC) × 100. Experiments were repeated 2 to 4 times. Mean values ±standard error of the mean are reported for 2 naive macaques (#419 and 421), an unvaccinated, infected macaque (#858) and for 3 vaccinated elite controllers (#5, #7, and #9).