Figure 3.
Temperature optimization. Yeasts were co-transformed with the indicated plasmids, grown initially on SD/Glucose(-UL) at RT, then replica plated to created matching plates for comparison of growth at 34 and 37ºC. Growth on SD/Galactose(-UL) but not SD/Glucose(-UL) is indicative of a protein-protein interaction. For each plasmid pairing, results for 3 of 5 independent transformants are shown. +, protein-protein interaction; -, no interaction.