FIG. 1.

Lipogenic activity of undifferentiated hASC mono-/cocultures (U), adipogenic differentiated mono-/cocultures (D), and endothelial monocultures (EC) were evaluated. (A) DNA content was determined at days 2, 6, and 14 of culture. All cultures proliferated to significant extent by day 14 (*p<0.05). Lipid was quantified (B) and day 14 Oil Red O stained positive in all differentiated cultures (C, D, G, H) compared to undifferentiated cultures (insets). Differentiated cultures accumulated more lipid than undifferentiated and endothelial cultures (*p<0.05). Day 6 differentiated monocultures and day 14 undifferentiated cultures also showed increases from previous time points (⁺p<0.05). Glut4 was positively stained in differentiated cultures via immunohistochemistry (E, F, I, J) compared to undifferentiated cultures (insets). Scale bars: (C, E, G, I) 500 μm (original magnification 4×); (D, F, H, J) 100 μm (original magnification 20×). hASC, human adipose-derived mesenchymal stem cell; Glut4, glucose transporter 4. Color images available online at www.liebertonline.com/tea