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Steady-State Kinetic Parameters
| Enzyme |
Km(dTDP-Quip3N) (mM) |
kcat (s−1) | kcat/Km (M−1 s−1) |
|---|---|---|---|
| TylM1 | 0.045 ± 0.004 | 0.172 ± 0.004 | (3.8 ± 0.3) × 103 |
| H123A Mutant Protein | 0.67 ± 0.05 | 0.046 ± 0.001 | 69 ± 5 |
| H123N Mutant Protein | 0.93 ± 0.08 | 0.0126 ± 0.0003 | 14 ± 1 |
