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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Anal Chem. 2010 Dec 30;83(3):856–865. doi: 10.1021/ac102461e

Figure 2.

Figure 2

(A) The molecular locations of MS2 CID fragmentation of the asymmetrical urea bond that is associated with PIC labeling of peptide amino termini. Proximal cleavage results in the loss of a phenylamine (PhA) moiety from the peptide, while fragmentation at the distal location results in the loss of the entire PIC moiety. (B) The predicted neutral mass losses, as a function of the precursor charge state, for peptides labeled with either 12C6 (PIC-L) or 13C6 (PIC-H) phenylisocyanate when fragmented by CID.