Figure 1.

Generation of GnRH neuron-specific Jak2 knock-out mouse. A, Exon 2 of Jak2 is flanked by two LoxP sites, and Cre recombinase expression was regulated by the mouse GnRH promoter. Primers used in the PCRs are marked by arrows. B, PCR of DNA isolated from various tissues of control and Jak2 G^−/− mice. C, Immunostaining of Jak2 in GnRH-GFP mice. Brain was sectioned at 10 μm thick from control (left) and Jak2 G^−/− mice (right). GnRH neurons shown here are localized in the vertical limb of the diagonal band. Red is Jak2 immunostaining. Green is GnRH neuron. Yellow is colocalization (arrow) of Jak2 and GnRH. Scale bar, 20 μm.