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. 1994 Mar 25;22(6):1037–1043. doi: 10.1093/nar/22.6.1037

Intermolecular disintegration and intramolecular strand transfer activities of wild-type and mutant HIV-1 integrase.

A Mazumder 1, A Engelman 1, R Craigie 1, M Fesen 1, Y Pommier 1
PMCID: PMC307927  PMID: 8152908

Abstract

We report the activities of HIV integrase protein on a novel DNA substrate, consisting of a pair of gapped duplex molecules. Integrase catalyzed an intermolecular disintegration reaction that requires positioning of a pair of the gapped duplexes in a configuration that resembles the intgration intermediate. However, the major reaction resulted from an intramolecular reaction involving a single gapped duplex, giving rise to a hairpin. Surprisingly, a deletion mutant of integrase that lacks both the amino and carboxyl terminal regions still catalyzed the intermolecular disintegration reaction, but supported only a very low level of the intramolecular reaction. The central core region of integrase is therefore sufficient to both bind the gapped duplex DNA and juxtapose a pair of such molecules through protein-protein interactions. We suggest that the branched DNA structures of the previously reported disintegration substrate, and the intermolecular disintegration substrate described here, assist in stabilizing protein-protein interactions that otherwise require the amino and carboxy terminal regions of integrase.

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Selected References

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