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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Hepatology. 2011 Apr;53(4):1323–1332. doi: 10.1002/hep.24203

Figure 2. FIB-γ is the major insoluble liver protein after FasL-mediated injury.

Figure 2

(A) Fragment sequences of FIB-γ obtained from N-terminal sequencing (underlined) and mass spectrometry (bold) confirm the identity of band #2 shown in Fig. 1B. (B) Prediction of mass spectrometric results was verified by immunoblotting using antibodies to FIB-γ and actin. The FIB-γ antibody recognized several bands in the FasL-treated liver (250-kDa and 100-kDa) that were not present in the untreated controls. These species correspond to bands 1 and 2 (Fig. 1B), respectively. The actin blot demonstrated elevated levels of actin protein in FasL-treated liver compared to untreated control. Formation of the K18 apoptotic fragment confirmed the hepatocyte FasL-induced apoptosis.