Table 2.
Maternal serum and liver retinol and retinyl ester levels under different regimens of dietary vitamin A intake
| Tissue and genotype | VitA-suff |
VitA-def |
||||
|---|---|---|---|---|---|---|
| Retinol | Retinyl ester | n | Retinol | Retinyl ester | n | |
| Serum | ||||||
| WT | 20.7 ± 3.4 | 1.1 (nd–9.3) | 6 | 32.2 ± 3.7 | nd | 4 |
| RBP−/− | 2.2 ± 0.3* | 0.5 (nd–10.0) | 8 | 1.4 ± 0.4* | nd | 7 |
| CMOI−/− | 17.2 ± 2.8* | 0.3 (nd–12.0) | 10 | 35.2 ± 4.4 | nd | 5 |
| CMOI−/−RBP−/− | 2.0 ± 0.3* | 0.3 (nd–2.5) | 17 | 1.5 ± 0.3* | nd | 11 |
| Liver | ||||||
| WT | 3.7 ± 1.2 | 270.3 ± 116.6 | 6 | 7.8 ± 4.5 | 295.7 ± 90.5 | 7 |
| RBP−/− | 6.9 ± 2.4* | 387.5 ± 100.0 | 7 | 2.6 ± 1.4* | 380.4 ± 78.5 | 6 |
| CMOI−/− | 10.1 ± 0.5* | 326.6 ± 50.1 | 4 | 9.4 ± 4.2 | 375.6 ± 68.3 | 6 |
| CMOI−/−RBP−/− | 4.5 ± 1.0 | 490.3 ± 144.3* | 6 | 4.2 ± 0.9 | 474.2 ± 162.7* | 6 |
Retinol and retinyl ester levels in serum (μg/dl) and liver (μg/g) were determined by reverse-phase HPLC. VitA-suff, vitamin A-sufficient maternal dietary regimen; VitA-def, vitamin A-deficient maternal dietary regimen; WT, wild type; nd, not detectable. See text and Supplemental Table S1 for details. Values are expressed as means ± sd, except for serum retinyl ester levels, which are expressed as geometric means with range of absolute values in parentheses. Statistical analysis as described in Materials and Methods.
*
P < 0.05 vs. corresponding WT.