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CKD stimulates myostatin expression in muscle. A) Myostatin mRNA in muscles of CKD mice was significantly higher vs. results in control mice (n=6/group). B) Top panel: representative Western blots of myostatin protein in gastrocnemius muscles of CKD or control mice. Bottom panel: quantification (n=6/group). C) Sections of TA muscles from control and CKD mice were immunostained with an anti-myostatin (red) and DAPI (blue). Scale bars = 20 μm. *P < 0.05.
