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. 2011 May;25(5):1653–1663. doi: 10.1096/fj.10-176917

Figure 3.

Figure 3.

Myostatin inhibition increased insulin/IGF-1 intracellular signaling pathway in muscle. A) Top panel: representative Western blot of p-Akt in muscle. Bottom panel: quantification of p-Akt/Akt ratio (n=7/group). B) Recombinant myostatin decreased Akt hosphorylation in C2C12 myotubes, and the anti-myostatin peptibody increased p-Akt. Western blot of p-Smad2 was used to document myostatin activity (n=3 repeated experiments). C, D) Myostatin inhibition increased FoxO1 (C) and FoxO3A (D) phosphorylation (n=7/group). Ctrl, control. *P < 0.05.