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. 2011 May;25(5):1653–1663. doi: 10.1096/fj.10-176917

Table 1.

RT-PCR primer sequences

mRNA RT-PCR primers
Forward Reverse
IL6 5′-GAGGATACCACTCCCAACAGACC-3′ 5′-AAGTGCATCATCGTTGTTCATACA-3′
TNF-α 5′-CATGAGCACAGAAAGCATGATCCG-3′ 5′-AAGCAGGAATGAGAAGAGGCTGAG-3′
Myostatin 5′-TGGCATTACTCAAAAGCAAAAAG-3′ 5′-CATCAATACTCTGCCAAATACCA-3′
TGF-β1 5′-TACCATGCCAACTTCTGTCTGGGA-3′ 5′-TGTGTTGGTTGTAGAGGGCAAGGA-3′
F4/80 5′-AAGCATCCGAGACACACACAGTCT-3′ 5′-TGACTGTACCCACATGGCTGATGA-3′
Myogenin 5′-ACAGCATCACGGTGGAGGATATGT-3′ 5′-CCCTGCTACAGAAGTGATGGCTTT-3′
MyoD 5′-ACGACTGCTTTCTTCACCACTCCT-3′ 5′-TCGTCTTAACTTTCTGCCACTCCG-3′
GAPDH 5′-ACCACCATGGAGAAGGCCGG-3′ 5′-CTCAGTGTAGCCCAAGATGC-3′
18S 5′-GAAACGGCTACCACATCCAAGG-3′ 5′-GTCCCTCTTAATCATGGCCTCAG-3′