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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 13;79(3):729–742. doi: 10.1111/j.1365-2958.2010.07481.x

Figure 2. Comparison of N. gonorrhoeae PhrB and E. coli PhrB.

Figure 2

A. Amino acid sequence alignment of N. gonorrhoeae (Gc) strain FA1090 PhrB and E. coli (Ec) PhrB. Identical residues are indicated by white letters boxed in grey while conserved residues are indicated by black letters boxed in grey. A black line indicates gonococcal residues 18–171 that are most similar (E-value of 2e−08) to the DNA photolyase superfamily pfam00875 and a grey line indicates gonococcal residues 206–426 that are most similar (E-value of 4e−47) to the flavin adenine dinucleotide (FAD) binding domain superfamily 7 pfam03441. Asterisks indicate identical or conserved residues that are involved in DNA binding (Weber, 2005). B. E. coli survival after UV irradiation. E. coli phrB strain CSR603 (Ec PhrB−) was complemented with either E. coli phrB (Ec PhrB−/pBlunt-phrBEc) or the gonococcal phrB (Ec PhrB−/pBlunt-phrBGc) on a high-copy plasmid (Sancar & Rupert, 1978b). Immediately following 0, 20, 40, and 60 joules/m2 UV irradiation, bacteria were either exposed to light (L) for 1 hour or kept in the dark (D). Survival relative to cells not treated with UV irradiation was calculated after growth at 37°C in the dark for 16–18 hours. Error bars represent the standard error of the mean of four independent experiments. Asterisks indicate P<0.05 as compared to exposed to light show as measured by two-tailed Student’s t-test.