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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 13;79(3):729–742. doi: 10.1111/j.1365-2958.2010.07481.x

Figure 3. Genome organization of tthe phrB locus, mutant and complement.

Figure 3

A. Chromosomal map of the phrB locus. In Gc strain FA1090 chromosome, phrB (black arrow) is located between NGO1706 and NGO1708 (large grey arrows). The phrB mutant strain contains a kanamycin transposon insertion (KmR, triangle) 227 bps from the 5′ end of the 1296 bp orf. B. The phrB complement strain. The phrB gene was cloned downstream of a dual taclac promoter (hatched box) (Mehr & Seifert, 1998). This construct was transformed into the phrB mutant and inserted into the neisserial intergenic complementation site (NICS) (Mehr & Seifert, 1998).