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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 13;79(3):729–742. doi: 10.1111/j.1365-2958.2010.07481.x

Figure 7. Analysis of PhrB DNA binding and bending in vitro.

Figure 7

A. Fluorescence anisotropy of PhrB plotted at increasing protein concentrations per amount of 30 bp dsDNA oligonucleotide substrate (o30dsDNA). Error bars represent the standard error of the mean for 3 independent experiments with a total of 6 replicates. B. Fluorescence anisotropy of PhrB plotted at increasing protein concentrations per amount of UV irradiated (1000 joules/m2) o30dsDNA oligonucleotide substrate. Error bars represent the standard error of the mean for 3 independent experiments with a total of 6 replicates. C. Protein duplex DNA bending assay. Shown is a (2.5 μg/ml) chloroquine gel of plasmid DNA (pCRBlunt2bp) incubated with topoisomerase I, DNA gyrase with or without the addition of topoisomerase I, and increasing concentrations of PhrB to DNA (1:1, 10:1, 100:1) with or without the addition of topoisomerase I. The direction of migration of less negatively supercoiled DNA (+) and more negatively supercoiled DNA (−) is indicated.