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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Cancer Res. 2011 Jan 6;71(5):1526–1532. doi: 10.1158/0008-5472.CAN-10-3069

Figure 2. Detection sensitivity and specificity.

Figure 2

(A) Left SERS signals from EGF-SERS (brown) and non-targeted SERS (pink) nanoparticles in Tu212 cells (1×104) spiked in 2ml mouse blood. Controls tested non-specific binding of EGF-SERS nanoparticles to healthy blood cells of mouse (blue) and human (green); Right: Comparing the SERS intensities for targeted and non-targeted nanoparticles in (A). (B) Left: SERS spectra of 10, 100, 500, and 1000 Tu212 cells labeled with EGF-SERS tags in 1×107 WBCs. Right: The correlation between relative SERS-signal intensity and the number of spiked Tu212 cells. (C) Left: SERS spectra of 1×104 Tu212, MDA-MB-231, H292, H460 cells in 5×106 WBCs. (D) Left: Examination of EGFR-expression by FACS. Right: Various levels of EGFR-expression measured by SERS (C) and fluorescence (D) intensities. Error bars represent the standard deviation of three replicates.