Table 2.
Strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strainsa | ||
| DH5α | hsdR recA endA | (Grant et al., 1990) |
| Tuner | OmpT− Lon− | Novagen |
| BL21 | OmpT− Lon− | (Rosenberg et al., 1987) |
| SF100 | ΔompT | (Baneyx and Georgiou, 1990) |
| AAEC185 | Δfim | (Blomfield et al., 1991) |
| ORN103 | Δfim | (Orndorff et al., 1985) |
| MM294ΔfimD | ΔfimD | (Shu Kin So and Thanassi, 2006) |
| Plasmids | ||
| pMON6235Δcat | vector, Para, Ampr | (Jones et al., 1997) |
| pMMB66 | vector, Ptac, Ampr | (Morales et al., 1991) |
| pMMB91 | vector, Ptac, Kanr | (Dodson et al., 1993) |
| pBAD18-cm | vector, Para, Clmr | (Guzman et al., 1995) |
| pBAD18-kan | vector, Para, Kanr | (Guzman et al., 1995) |
| pMJ3 | PapC with C-terminal His-tag in pMON6235Δcat | (Thanassi et al., 1998) |
| pM05 | PapC F3A in pMJ3 | (Ng et al., 2004) |
| pTN5 | PapC C70A in pMJ3 | (Ng et al., 2004) |
| pTN26 | PapC Δ77–86 in pMJ3 | This study |
| pMJ2 | ΔpapC pap operon in pACYC184, Ptrc, Tetr | (Thanassi et al., 1998) |
| pPAP58 | PapDJKEFG in pMMB91 | (Hultgren et al., 1989) |
| pJP1 | PapDG in pMMB91 | (Dodson et al., 1993) |
| pETS4 | FimD with C-terminal His-tag in pMMB66 | (Saulino et al., 1998) |
| pETS7 | FimD with C-terminal His-tag in pBAD18-cm | (Saulino et al., 2000) |
| pAN2 | FimD (no His-tag) in pMMB91 | (Saulino et al., 1998) |
| pNH227 | FimD F4A in pETS4 | This study |
| pIT2 | FimD F4A in pETS7 | This study |
| pNH238 | FimD F4A in pAN2 | This study |
| pNH228 | FimD C63A in pETS4 | This study |
| pIT4 | FimD C63A in pETS7 | This study |
| pNH236 | FimD Δ70–79 in pETS4 | This study |
| pIT12 | FimD Δ70–79 in pETS7 | This study |
| pNH239 | FimD Δ70–79 in pAN2 | This study |
| pNH222 | FimCGH in pBAD18-kan | This study |
| pNH235 | FimCFGH in pBAD18-cm | This study |
| pETS1007 | FimCH in pMMB66 | (Saulino et al., 2000) |
| pNH221 | FimC (no His-tag) in pBAD18-cm | This study |
| pETS1000 | FimC with C-terminal His-tag in pMON6235Δcat, Specr | (Saulino et al., 1998) |
| pHJ20 | FimH in pMMB66 | (Jones et al., 1995) |
| pETS2A | FimG in pMMB66 | (Saulino et al., 1998) |
a
All strains are E. coli K-12, except BL21 and Tuner, which are E. coli B.
Ampr, ampicillin resistance; Kanr, kanamycin resistance; Clmr, chloramphenicol resistance; Tetr, tetracycline resistance; Specr, spectinomycin resistance; Para, arabinose-inducible promoter, Ptac or Ptrc, IPTG-inducible promoter.