Table 3.
Mutation | Sequence of QuikChange forward primer (5’-3’)a |
---|---|
PapC Δ77–86 | CCGCAGGCCTGTCTGACATCAGATATGGTCGATAAAGTTG |
FimD F4A | CTGCCGACCTCTATGCTAATCCGCGC |
FimD C63A | GGGATTGTTCCCGCCCTGACACGCGCG |
FimD Δ70–79 | GCGCGCAACTCGCCGGTATGAATCTGC |
The reverse primer is the reverse complement of the forward primer.