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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Hepatology. 2011 Mar;53(3):1007–1019. doi: 10.1002/hep.24162

Figure 3. Kinetic analyses of the impact of cotransplantation with HSC on accumulation of MDSC and Treg cells.

Figure 3

(A) Cryostat sections of islet grafts harvested on POD7, 14 and >90 (long-term, LT) (n=3 in each group) were double stained with anti-CD11b (green) and -CD11c (red) or -CD4 (red) and -Fop3(green) mAbs, and evaluated under a fluorescent microscope. The double positive cells were counted in total 10 high power fields (hpf) that were randomly selected in each graft. The data are expressed as mean double positive cells/graft ± 1SD. (B and C) Lymphocytes isolated from draining LN or spleen at the indicated time points (n=3 in each group) were double stained with anti-CD11b and -CD11c or –CD4 and –Foxp3 mAbs, and analyzed by flow cytometry. The absolute numbers of CD11b+CD11c− and CD4+Foxp3+ cells are calculated. The data are expressed as mean cells/LN or spleen ± 1SD.