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. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: Hepatology. 2011 Mar;53(3):1007–1019. doi: 10.1002/hep.24162

(A) Administration of H-MC attenuates liver damage. DC or H-MC (1.5 × 10⁶) that had been pulsed with OVA were intravenously injected into OVA-HEP mice whose liver injury was induced by adoptive transfer of T cells from OT-1 (5 × 10⁶) and OT-II (2 × 10⁶) mice. Serum was obtained on day 3 post cell injection, and tested for ALT with specific kit. (B) Administration of H-MC leads to less antigen specific CD8⁺ infiltrating cells in the liver. The animals were sacrificed on day 6 post cell transfer. The liver cryostat sections were histochemically stained with anti-CD4 or -CD8 mAb, and examined by microscopy. (C) A total of 30 high power fields (hpf) were randomly selected in each liver (n=3), and counted for CD4⁺ and CD8⁺ cells. The data are expressed as cell number/hpf ± 1SD. (D) Cotransplantation with H-MC effectively protects islet allografts. 300 BALB/c islets were mixed with 2, 5 × 10⁶ of H-MC or DC generated from B6 BM by co-culture with HSC either from B6, BALB/c or C3H strain, and transplanted into diabetic B6 recipients. Islet allografts alone served as controls. The islet graft survival was monitored by blood glucose level as described in Methods.

(A) Administration of H-MC attenuates liver damage. DC or H-MC (1.5 × 10⁶) that had been pulsed with OVA were intravenously injected into OVA-HEP mice whose liver injury was induced by adoptive transfer of T cells from OT-1 (5 × 10⁶) and OT-II (2 × 10⁶) mice. Serum was obtained on day 3 post cell injection, and tested for ALT with specific kit. (B) Administration of H-MC leads to less antigen specific CD8⁺ infiltrating cells in the liver. The animals were sacrificed on day 6 post cell transfer. The liver cryostat sections were histochemically stained with anti-CD4 or -CD8 mAb, and examined by microscopy. (C) A total of 30 high power fields (hpf) were randomly selected in each liver (n=3), and counted for CD4⁺ and CD8⁺ cells. The data are expressed as cell number/hpf ± 1SD. (D) Cotransplantation with H-MC effectively protects islet allografts. 300 BALB/c islets were mixed with 2, 5 × 10⁶ of H-MC or DC generated from B6 BM by co-culture with HSC either from B6, BALB/c or C3H strain, and transplanted into diabetic B6 recipients. Islet allografts alone served as controls. The islet graft survival was monitored by blood glucose level as described in Methods.