Table 1.
Kinetic Parameter | T/Ab | U/A | 5-FU/A |
---|---|---|---|
kex (s−1) | 0.63 ± 0.02 | 6.3 ± 0.1 | 126 ± 8 |
kint × 10−6 (s−1) | 0.02 ± 0.03 | 0.4 ± 0.2 | 14 ± 3 |
kop (s−1) | 35 ± 6 | 200 ± 25 | 440 ± 20 |
kcl × 10−6 (s−1) | 1.8 ± 0.3 | 7 ± 3 | 37 ± 7 |
αKop × 106 | 20 ± 4 | 27 ± 11 | 12 ± 2 |
Abbreviations: 5-FU, 5-fluorouracil; U, uracil; A, adenine; T, thymine.
Kinetic parameters for the T•A base-pair were previously reported (13).