Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 22;79(4):872–881. doi: 10.1111/j.1365-2958.2010.07494.x

Fig. 1. Yeast two-hybrid analysis of ArsD mutants exhibiting increased interaction with ArsA.

Fig. 1

S. cerevisiae strain AH109 bearing pBD-k-D wild typeArsD or mutants (indicated at left) and either pAD-A (A) or pAD-D (B) plasmids was grown in SD medium overnight and inoculated with 10-fold serial dilutions on SD agar plates lacking histidine and supplemented with the indicated concentrations of 3-AT. The plates were incubated at 30 °C for 3 days and scored for growth.