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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 22;79(4):872–881. doi: 10.1111/j.1365-2958.2010.07494.x

Fig. 4. Yeast two-hybrid analysis of interaction between ArsD lysine mutants and ArsA or wild type ArsD.

Fig. 4

S. cerevisiae strain AH109 bearing pBD-k-D wild type or lysine mutants (indicated at left) and either pAD-A (A) or pAD-D (B) plasmids was grown in SD medium overnight and inoculated on agar plates with SD lacking histidine with 10-fold serial dilutions. Plates were incubated at 30 °C for 3 days and scored for growth. As a negative control, the top row contained yeast cells co-transformed with vector plasmids pGBT9 and pACT2.