Table1.
Summary of ArsD mutants
| Residue | Location | Mutations | Phenotype/Interaction with ArsA |
Residues in 100 closest homologues |
|---|---|---|---|---|
| Cys-12 | Metal binding loop | C12A | Inactive | Cys |
| Cys-13 | Metal binding loop | C13A | Inactive | Cys |
| Ser-14 | Metal binding loop | S14R | Increased | Ser |
| Val-17 | Metal binding loop | V17A | Decreased | Val |
| Cys-18 | Metal binding loop | C18S | Inactive | Cys |
| Thr-20 | Metal binding loop | T20I | Increased | Thr/Ser/Val/Ala/Pro |
| Val-22 | Metal binding loop | V22A | Decreased | Val/Thr |
| Gln-24 | Helix 1 | Q24L | Increased | Gln/Pro |
| Val-27 | Helix 1 | V27D | Decreased | Val/Thr |
| Asp-28 | Helix 1 | D28T/V | Increased | Asp/Asn/Thr/Ser/Ala |
| Thr-31 | Helix 1 | T31A | Increased | Thr/Ala |
| Gln-34 | Helix 1 | Q34R | Increased | Asn/Asp/Glu |
| Lys-37 | Helix 1 | K37A/R | Decreased/None | Lys/Arg |
| Gln-38 | Helix 1 | Q38R | Increased | Gln/Ser/Thr/Lys |
| Gln-51 | Loop | Q51H | Decreased | Gln/Glu |
| Phe-55 | Helix 2 | F55L | Decreased | Phe |
| Val-61 | Helix 3 | V61A | Increased | Val/Ala |
| Lys-62 | Helix 3 | K62A/R | Decreased/None | Lys |