GluR1 membrane insertion in dorsal horn neurons 24 h after CFA injection. (A) Surface expression of GluR1 in dorsal horn neurons 24 h after CFA or saline injection. Top, representative Western blot; bottom, statistical summary of the densitometric analysis. The level of sample loaded for the total (T) expression was 10% of that for the biotinylated surface (S) expression. * p < 0.05 versus the saline-treated group. β-actin, an unbiotinylated intracellular protein, was used as a control. (B) Expression of GluR1 in the synaptosomal fraction from dorsal horn 1 day after CFA or saline injection. Top, representative Western blot; bottom, statistical summary of the densitometric analysis. N-cadherin, a membrane marker, was used as a control.